Directed Electronics equalizer 2500 Guide de l'utilisateur télécharger pdf (Page 38)

Perform a HiSeq v4 Run

Part # 15035786 Rev. D

4 Slide the reagent rack out of the reagent compartment using the rack handle.

5 Remove the caps from each reagent tube and place the tube onto the rack in the

associated numbered position or matching label color.

Position

Reagent Description

FDR Fast Denaturation Reagent (contains formamide)

HP9 * Index Sequencing Primer i5

HP12 Index Sequencing Primer i7

Table 2 Single-Read Flow Cells

* HP9 is required for dual-indexed runs only. If HP9 is not used, load a 15 ml conical tube with 10 ml

laboratory-grade water in position 16.

Position

Reagent Description

FRM * Fast Resynthesis Mix

FDR Fast Denaturation Reagent (contains formamide)

HP12 Index Sequencing Primer i7

Table 3 Paired-End Flow Cells

* Load FRM in position 10 for dual-indexed runs on a paired-end flow cell. FRM is required in position

10 for all paired-end runs regardless of indexing options.

6 If you are performing a single-read run, proceed with the following steps to return the

rack to the reagent compartment. Otherwise, proceed to loading paired-end reagents.

7 Place 15 ml conical tubes filled with 10 ml laboratory-grade water in unused positions

on the paired-end rack.

8 Slide the reagent rack into the reagent compartment, aligning the rack with the raised

guide on the floor of the compartment.

9 If you are performing a single-read run, lower the sippers into the paired-end reagent

tubes as follows:

a Pull the handle towards you and then lower the handle.

b Visually inspect the sippers to make sure that they do not bend as they lower into

the tubes.

c Release the handle into the slot on the bottom end of the groove.

10 Select Next.

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Directed Electronics equalizer 2500 Guide de l'utilisateur Page 38